Abstract. The stable isotopic compositions of nitrate in precipitation (wet deposition) and groundwater (spring, lake, and stream water) were determined for the island of Rishiri, Japan, so as to use the ¹⁷O anomalies (Δ¹⁷O) to trace the fate of atmospheric nitrate that had deposited onto the island ecosystem, which is a representative background forest ecosystem for eastern Asia. The deposited nitrate had large ¹⁷O anomalies with Δ¹⁷O values ranging from +20.8‰ to +34.5‰ (n = 32) with +26.2‰ being the annual average. The maximum Δ¹⁷O value of +34.5‰, obtained for precipitation on the 23rd to 24th of February 2007, was an extraordinarily large value among values for all samples of precipitation in Rishiri. Most nitrate in the sample might have been produced via NO₃ radical in a highly polluted air mass that had been supplied from megacities on the eastern coast of the Asian continent. On the other hand, nitrate in groundwater had small Δ¹⁷O values ranging from +0.9‰ to 3.2‰ (n = 19), which corresponds to an mixing ratio of atmospheric nitrate to total nitrate of (7.4±2.6)%. Comparing the inflow and outflow of atmospheric nitrate in groundwater within the island, we estimated that the direct drainage accounts for (8.8±4.6)% of atmospheric nitrate that has deposited on the island and that the residual portion has undergone biological processing before being exported from the forest ecosystem.